Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2760579..2761495 Replicon chromosome
Accession NZ_CP106671
Organism Bacillus subtilis strain SRCM116301

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag N7980_RS14120 Protein ID WP_003244695.1
Coordinates 2760579..2761325 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag N7980_RS14125 Protein ID WP_003232646.1
Coordinates 2761325..2761495 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7980_RS14095 (2755661) 2755661..2755807 - 147 WP_120363335.1 hypothetical protein -
N7980_RS14100 (2755908) 2755908..2756858 - 951 WP_041344800.1 ring-cleaving dioxygenase -
N7980_RS14105 (2757246) 2757246..2758562 + 1317 WP_041850924.1 serine/threonine exchanger -
N7980_RS14110 (2758838) 2758838..2759455 + 618 WP_003218470.1 DUF47 domain-containing protein -
N7980_RS14115 (2759468) 2759468..2760469 + 1002 WP_003232642.1 inorganic phosphate transporter -
N7980_RS14120 (2760579) 2760579..2761325 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
N7980_RS14125 (2761325) 2761325..2761495 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
N7980_RS14130 (2761581) 2761581..2761718 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
N7980_RS14135 (2761755) 2761755..2762648 - 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
N7980_RS14140 (2762661) 2762661..2762924 - 264 WP_003232653.1 phage holin -
N7980_RS14145 (2762937) 2762937..2763206 - 270 WP_003232655.1 hemolysin XhlA family protein -
N7980_RS14150 (2763259) 2763259..2764098 - 840 WP_041850926.1 phage-like element PBSX protein XepA -
N7980_RS14155 (2764142) 2764142..2764306 - 165 WP_041850927.1 XkdX family protein -
N7980_RS14160 (2764303) 2764303..2764632 - 330 WP_041850928.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T259620 WP_003244695.1 NZ_CP106671:2760579-2761325 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT259620 WP_003232646.1 NZ_CP106671:2761325-2761495 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References