Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2091956..2092546 Replicon chromosome
Accession NZ_CP106658
Organism Klebsiella michiganensis strain 8BG

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag N7918_RS09990 Protein ID WP_088168729.1
Coordinates 2092214..2092546 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag N7918_RS09985 Protein ID WP_004852307.1
Coordinates 2091956..2092213 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7918_RS09960 (N7918_09960) 2087212..2087817 - 606 WP_032720304.1 glutathione S-transferase family protein -
N7918_RS09965 (N7918_09965) 2087997..2088923 + 927 WP_014838892.1 LysR substrate-binding domain-containing protein -
N7918_RS09970 (N7918_09970) 2088961..2089959 - 999 WP_014229982.1 aldo/keto reductase -
N7918_RS09975 (N7918_09975) 2090060..2090974 + 915 WP_262191307.1 LysR family transcriptional regulator -
N7918_RS09980 (N7918_09980) 2091165..2091245 - 81 Protein_1958 molybdopterin-binding protein -
N7918_RS09985 (N7918_09985) 2091956..2092213 + 258 WP_004852307.1 antitoxin Antitoxin
N7918_RS09990 (N7918_09990) 2092214..2092546 + 333 WP_088168729.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N7918_RS10000 (N7918_10000) 2092869..2094326 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
N7918_RS10010 (N7918_10010) 2094689..2095948 + 1260 WP_032720308.1 integrase arm-type DNA-binding domain-containing protein -
N7918_RS10015 (N7918_10015) 2096315..2096614 - 300 WP_071785591.1 microcin E492 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB 2082250..2117722 35472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11788.74 Da        Isoelectric Point: 10.1863

>T259593 WP_088168729.1 NZ_CP106658:2092214-2092546 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVPLEGAGIKTTGVIRCDQPITI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT259593 WP_004852307.1 NZ_CP106658:2091956-2092213 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References