Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3778..4514 Replicon plasmid pKP5076-1
Accession NZ_CP106655
Organism Klebsiella pneumoniae strain KP5076

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag N7990_RS25975 Protein ID WP_003026803.1
Coordinates 3778..4260 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag N7990_RS25980 Protein ID WP_003026799.1
Coordinates 4248..4514 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7990_RS25960 (N7990_25960) 603..1307 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
N7990_RS25965 (N7990_25965) 1513..2145 + 633 WP_001567369.1 hypothetical protein -
N7990_RS25970 (N7990_25970) 2174..3577 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
N7990_RS25975 (N7990_25975) 3778..4260 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
N7990_RS25980 (N7990_25980) 4248..4514 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
N7990_RS25985 (N7990_25985) 4759..5193 - 435 WP_001567367.1 cell envelope integrity protein TolA -
N7990_RS25990 (N7990_25990) 5240..5788 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
N7990_RS25995 (N7990_25995) 6280..7400 + 1121 WP_087759866.1 IS3-like element ISKpn34 family transposase -
N7990_RS26000 (N7990_26000) 7507..7815 + 309 WP_017896554.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..190782 190782
- inside IScluster/Tn - - 603..7400 6797


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T259585 WP_003026803.1 NZ_CP106655:c4260-3778 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT259585 WP_003026799.1 NZ_CP106655:c4514-4248 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References