Detailed information of TA system TA259536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 528071..529096
• Replicon: plasmid p_unnamed1
• Accession: NZ_CP104972
• Organism: Phyllobacterium zundukense strain A18/3m

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: N8E88_RS10045
• Protein ID: WP_262291626.1
• Coordinates: 528071..528640 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: N8E88_RS10050
• Protein ID: WP_262292402.1
• Coordinates: 528644..529096 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N8E88_RS10020 (N8E88_10030)	524302..525000	+	699	WP_262291621.1	AraC family transcriptional regulator	-
N8E88_RS10025 (N8E88_10035)	525327..526010	-	684	WP_262291622.1	AraC family transcriptional regulator	-
N8E88_RS10030 (N8E88_10040)	526022..526282	+	261	WP_262291623.1	hypothetical protein	-
N8E88_RS10035 (N8E88_10045)	526655..527458	+	804	WP_262291624.1	SDR family oxidoreductase	-
N8E88_RS10040 (N8E88_10050)	527619..527921	+	303	WP_262291625.1	DNA-binding protein	-
N8E88_RS10045 (N8E88_10055)	528071..528640	-	570	WP_262291626.1	PIN domain-containing protein	Toxin
N8E88_RS10050 (N8E88_10060)	528644..529096	-	453	WP_262292402.1	excisionase family DNA-binding protein	Antitoxin
N8E88_RS10055 (N8E88_10065)	529421..529722	+	302	Protein_524	pyridine nucleotide-disulfide oxidoreductase	-
N8E88_RS10060 (N8E88_10070)	529897..530094	-	198	WP_262291627.1	hypothetical protein	-
N8E88_RS10065 (N8E88_10075)	530152..530952	-	801	WP_262291628.1	methyltransferase domain-containing protein	-
N8E88_RS10070 (N8E88_10080)	531373..533847	-	2475	WP_262291629.1	SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	flhA / fliQ / fliP / htpB	1..990469	990469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• SinI-like, DNA-binding domain (80-127)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21509.57 Da
• Isoelectric Point: 5.8103

>T259536 WP_262291626.1 NZ_CP104972:c528640-528071 [Phyllobacterium zundukense]
MGTFTAFLDASVLYPAPLRDLLLELAVSDLYRAKWSNTVHDEWINALLTSRDDLTRERLERTRHLMNAHVRDALVTDFDQ
LIGILQLPDPDDRHVLAAAIKGRADLIVTANLKDFPHSQLAPWGIEAQHPDEFLTHQFHLSQPQFLAAIKAVRLRLKNPP
KTVEDYLDTLRAQGLLATVNEIQAFDQFI

Nucleotide

• Length: 570 bp

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16505.96 Da
• Isoelectric Point: 5.9534

>AT259536 WP_262292402.1 NZ_CP104972:c529096-528644 [Phyllobacterium zundukense]
MTAALTTNPPSKDDVELARASGQRLAPYARKGLALTFRVRDADNEEAIELPAGAVKLLMAVLEDMASGRAVTIVPQNAEL
TTQQAADVLNVSRPFLIGLLDERKLPFRMVGTHRRVRFEDVLNYKEAIDTERRKVLGQLAAEAQELGMGY

Nucleotide

• Length: 453 bp