Detailed information of TA system TA259528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 88328..89786
• Replicon: plasmid p_unnamed1
• Accession: NZ_CP104967
• Organism: Phyllobacterium sp. A18/5-2

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: N8E89_RS18910
• Protein ID: WP_262263348.1
• Coordinates: 88328..89233 (-)
• Length: 302 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: N8E89_RS18915
• Protein ID: WP_262263349.1
• Coordinates: 89223..89786 (-)
• Length: 188 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N8E89_RS18895 (N8E89_18890)	83760..85097	-	1338	WP_262262769.1	nucleotide sugar dehydrogenase	-
N8E89_RS18900 (N8E89_18895)	85479..86817	+	1339	Protein_93	adenylate/guanylate cyclase domain-containing protein	-
N8E89_RS18905 (N8E89_18900)	86835..87773	+	939	WP_262263347.1	polysaccharide lyase	-
N8E89_RS18910 (N8E89_18905)	88328..89233	-	906	WP_262263348.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
N8E89_RS18915 (N8E89_18910)	89223..89786	-	564	WP_262263349.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
N8E89_RS18920 (N8E89_18915)	90207..90416	-	210	Protein_97	type II toxin-antitoxin system VapB family antitoxin	-
N8E89_RS18925 (N8E89_18920)	90954..92840	+	1887	WP_262262770.1	ABC transporter ATP-binding protein	-
N8E89_RS18930 (N8E89_18925)	92837..94759	+	1923	WP_262262771.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliP / flgI / fliI / motA / gmd / htpB / flhA / fliQ	1..1594332	1594332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-270)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (2-37)

Sequences

Toxin

Protein

• Length: 302 a.a.
• Molecular weight: 33881.79 Da
• Isoelectric Point: 5.9604

>T259528 WP_262263348.1 NZ_CP104967:c89233-88328 [Phyllobacterium sp. A18/5-2]
MAGEPKKNVGASVRARLLDRSRAERTDFQILLTRYALERFLYRLSLSAHRDRFILKGAMLFVAWVADPFRPTRDLDLLGY
GENSPRAIAETFHAICTQPVEDDGVVFDLDGLEAAPIREEVEYGGVRVRTTATIAGARIPIQVDIGFGDAVTPGPVDIEY
PSLLDAPAPRLRAYPVATVIAEKFEALVTLGMANSRLKDFYDLWLIAETFELERSTLAEAARQTFARRGTDFRKERPMGL
SDAYAEVWDRQWRAFLGRERMAAAPPRLAAVVTDLGRLLLPLMAQTDGDWRWKPREGWIQS

Nucleotide

• Length: 906 bp

Antitoxin

Protein

• Length: 188 a.a.
• Molecular weight: 20971.24 Da
• Isoelectric Point: 9.7605

>AT259528 WP_262263349.1 NZ_CP104967:c89786-89223 [Phyllobacterium sp. A18/5-2]
MLRLKDFAAHGIGSETLARLVRDEEVVRPARGLYQLPDAAADARHVLAEASALVPKGIVCLTSALQFHELTLQMPSAVWM
AIDRTAWRPKIDYPPIRFVRFTGAALTEGVERHRIEGVEVPITNPARSIVDCFRYRTKVGLDVAMEGLREGLRRRKTTSD
ELWAYAKRARIWSTMRPYVEATVADGG

Nucleotide

• Length: 564 bp