Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1324759..1325675 Replicon chromosome
Accession NZ_CP104878
Organism Bacillus subtilis strain 4ZT

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag N8A75_RS06950 Protein ID WP_003244695.1
Coordinates 1324929..1325675 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag N8A75_RS06945 Protein ID WP_003232646.1
Coordinates 1324759..1324929 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A75_RS06910 (1321619) 1321619..1321948 + 330 WP_046380959.1 XkdW family protein -
N8A75_RS06915 (1321945) 1321945..1322109 + 165 WP_014479563.1 XkdX family protein -
N8A75_RS06920 (1322156) 1322156..1322995 + 840 WP_046380960.1 phage-like element PBSX protein XepA -
N8A75_RS06925 (1323048) 1323048..1323317 + 270 WP_015252265.1 hemolysin XhlA family protein -
N8A75_RS06930 (1323330) 1323330..1323593 + 264 WP_003232653.1 phage holin -
N8A75_RS06935 (1323606) 1323606..1324499 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
N8A75_RS06940 (1324536) 1324536..1324673 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
N8A75_RS06945 (1324759) 1324759..1324929 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
N8A75_RS06950 (1324929) 1324929..1325675 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
N8A75_RS06955 (1325785) 1325785..1326786 - 1002 WP_003232642.1 inorganic phosphate transporter -
N8A75_RS06960 (1326799) 1326799..1327416 - 618 WP_003218470.1 DUF47 domain-containing protein -
N8A75_RS06965 (1327692) 1327692..1329008 - 1317 WP_046380961.1 serine/threonine exchanger -
N8A75_RS06970 (1329397) 1329397..1330347 + 951 WP_014479571.1 ring-cleaving dioxygenase -
N8A75_RS06975 (1330455) 1330455..1330550 + 96 Protein_1310 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T259438 WP_003244695.1 NZ_CP104878:c1325675-1324929 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT259438 WP_003232646.1 NZ_CP104878:c1324929-1324759 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References