Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519294..519930 Replicon chromosome
Accession NZ_CP104878
Organism Bacillus subtilis strain 4ZT

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag N8A75_RS02655 Protein ID WP_003156187.1
Coordinates 519580..519930 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag N8A75_RS02650 Protein ID WP_003225183.1
Coordinates 519294..519575 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A75_RS02630 (515653) 515653..516252 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
N8A75_RS02635 (516347) 516347..516712 + 366 WP_015252768.1 holo-ACP synthase -
N8A75_RS02640 (516878) 516878..517894 + 1017 WP_080019556.1 outer membrane lipoprotein carrier protein LolA -
N8A75_RS02645 (518009) 518009..519178 + 1170 WP_015252766.1 alanine racemase -
N8A75_RS02650 (519294) 519294..519575 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
N8A75_RS02655 (519580) 519580..519930 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N8A75_RS02660 (520045) 520045..520869 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
N8A75_RS02665 (520874) 520874..521239 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
N8A75_RS02670 (521243) 521243..521644 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
N8A75_RS02675 (521656) 521656..522663 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
N8A75_RS02680 (522725) 522725..523054 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
N8A75_RS02685 (523051) 523051..523533 + 483 WP_003234299.1 anti-sigma B factor RsbW -
N8A75_RS02690 (523499) 523499..524287 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
N8A75_RS02695 (524287) 524287..524886 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T259437 WP_003156187.1 NZ_CP104878:519580-519930 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT259437 WP_003225183.1 NZ_CP104878:519294-519575 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References