Detailed information of TA system TA259429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1715418..1717113
• Replicon: chromosome
• Accession: NZ_CP104870
• Organism: Pseudomonas aeruginosa strain PALA9

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: PALA9_RS07980
• Protein ID: WP_124180049.1
• Coordinates: 1715418..1716287 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: PALA9_RS07985
• Protein ID: WP_003112902.1
• Coordinates: 1716280..1717113 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PALA9_RS07955 (PALA9_01580)	1711426..1712277	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
PALA9_RS07960 (PALA9_01581)	1712318..1713325	+	1008	WP_023083382.1	ABC transporter substrate-binding protein	-
PALA9_RS07965 (PALA9_01582)	1713322..1714098	+	777	WP_003112898.1	ABC transporter permease	-
PALA9_RS07970 (PALA9_01583)	1714104..1714865	+	762	WP_023083383.1	SDR family oxidoreductase	-
PALA9_RS07975 (PALA9_01584)	1714879..1715409	+	531	WP_003112900.1	cupin domain-containing protein	-
PALA9_RS07980 (PALA9_01585)	1715418..1716287	+	870	WP_124180049.1	alpha/beta hydrolase	Toxin
PALA9_RS07985 (PALA9_01586)	1716280..1717113	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
PALA9_RS07990 (PALA9_01587)	1717100..1717897	+	798	WP_003112903.1	SDR family oxidoreductase	-
PALA9_RS07995 (PALA9_01588)	1717890..1719572	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
PALA9_RS08000 (PALA9_01589)	1719583..1720386	+	804	WP_003130116.1	aspartate dehydrogenase	-
PALA9_RS08005 (PALA9_01590)	1720398..1721882	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30377.56 Da
• Isoelectric Point: 6.5894

>T259429 WP_124180049.1 NZ_CP104870:1715418-1716287 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNAEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT259429 WP_003112902.1 NZ_CP104870:1716280-1717113 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Antitoxin

• AlphaFold DB: Q9HYA1