259402

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2719914..2720956 Replicon chromosome
Accession NZ_CP104866
Organism Pseudomonas aeruginosa strain PALA4

Toxin (Protein)


Gene name PP_4152 Uniprot ID -
Locus tag PALA4_RS12825 Protein ID WP_003153636.1
Coordinates 2720381..2720956 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID I3TV68
Locus tag PALA4_RS12820 Protein ID WP_003050245.1
Coordinates 2719914..2720384 (+) Length 157 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA4_RS12785 (PALA4_02533) 2715297..2716727 - 1431 WP_009516218.1 TIGR03752 family integrating conjugative element protein -
PALA4_RS12790 (PALA4_02534) 2716717..2717625 - 909 WP_009516219.1 TIGR03749 family integrating conjugative element protein -
PALA4_RS12795 (PALA4_02535) 2717622..2718314 - 693 WP_009516220.1 TIGR03746 family integrating conjugative element protein -
PALA4_RS12800 (PALA4_02536) 2718311..2718709 - 399 WP_009516221.1 TIGR03750 family conjugal transfer protein -
PALA4_RS12805 (PALA4_02537) 2718721..2719080 - 360 WP_003090173.1 TIGR03745 family integrating conjugative element membrane protein -
PALA4_RS12810 (PALA4_02538) 2719097..2719330 - 234 WP_003050225.1 TIGR03758 family integrating conjugative element protein -
PALA4_RS12815 (PALA4_02539) 2719327..2719710 - 384 WP_003090167.1 RAQPRD family integrative conjugative element protein -
PALA4_RS12820 (PALA4_02540) 2719914..2720384 + 471 WP_003050245.1 helix-turn-helix domain-containing protein Antitoxin
PALA4_RS12825 (PALA4_02541) 2720381..2720956 + 576 WP_003153636.1 PIN domain-containing protein Toxin
PALA4_RS12830 (PALA4_02542) 2720974..2721888 + 915 WP_003050256.1 AAA family ATPase -
PALA4_RS12835 (PALA4_02543) 2721885..2722355 + 471 WP_003090160.1 type III CBASS phage resistance system CD-NTase-associated protein Cap8 -
PALA4_RS12840 (PALA4_02544) 2722352..2722852 + 501 WP_003090159.1 type III CBASS phage resistance system CD-NTase-associated protein Cap7 -
PALA4_RS12845 (PALA4_02545) 2722852..2723754 + 903 WP_003090158.1 CBASS oligonucleotide cyclase -
PALA4_RS12850 (PALA4_02546) 2723793..2724518 + 726 WP_003050273.1 CBASS effector endonuclease NucC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2648114..2772559 124445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-114)

Antitoxin

Helix-turn-helix domain, group 17

(85-133)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21629.78 Da        Isoelectric Point: 5.9995

>T259402 WP_003153636.1 NZ_CP104866:2720381-2720956 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Download         Length: 576 bp

Antitoxin


Download         Length: 157 a.a.        Molecular weight: 17245.62 Da        Isoelectric Point: 6.3803

>AT259402 WP_003050245.1 NZ_CP104866:2719914-2720384 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Download         Length: 471 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I3TV68

References