259360

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 32887..34024 Replicon plasmid pNY13412
Accession NZ_CP104857
Organism Enterococcus faecalis strain NY13412

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag N7M47_RS14965 Protein ID WP_002332783.1
Coordinates 32887..33750 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag N7M47_RS14970 Protein ID WP_002326825.1
Coordinates 33752..34024 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7M47_RS14945 (N7M47_14950) 29021..29305 + 285 WP_000922261.1 hypothetical protein -
N7M47_RS14950 (N7M47_14955) 29312..31264 + 1953 WP_128701263.1 insertion element protein -
N7M47_RS14955 (N7M47_14960) 31336..31833 - 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -
N7M47_RS14960 (N7M47_14965) 32130..32447 - 318 WP_002326830.1 hypothetical protein -
N7M47_RS14965 (N7M47_14970) 32887..33750 - 864 WP_002332783.1 zeta toxin family protein Toxin
N7M47_RS14970 (N7M47_14975) 33752..34024 - 273 WP_002326825.1 antitoxin Antitoxin
N7M47_RS14975 (N7M47_14980) 34042..34257 - 216 WP_001835296.1 peptide-binding protein -
N7M47_RS14980 (N7M47_14985) 34349..35245 - 897 WP_002326827.1 ParA family protein -
N7M47_RS14985 (N7M47_14990) 35348..35608 - 261 Protein_42 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
N7M47_RS14990 (N7M47_14995) 35778..36515 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
N7M47_RS14995 (N7M47_15000) 36640..36723 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
N7M47_RS15000 36772..36857 - 86 Protein_45 peptide-binding protein -
N7M47_RS15005 (N7M47_15005) 37267..38061 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
N7M47_RS15010 (N7M47_15010) 38154..38696 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrG / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') - 1..49839 49839
- inside IScluster/Tn dfrG / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') - 21734..44160 22426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T259360 WP_002332783.1 NZ_CP104857:c33750-32887 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT259360 WP_002326825.1 NZ_CP104857:c34024-33752 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References