Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1316344..1317260 Replicon chromosome
Accession NZ_CP104855
Organism Bacillus subtilis strain TY-1

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag N6G78_RS06960 Protein ID WP_003244695.1
Coordinates 1316514..1317260 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag N6G78_RS06955 Protein ID WP_003232646.1
Coordinates 1316344..1316514 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6G78_RS06920 (N6G78_06920) 1313203..1313532 + 330 WP_015483132.1 XkdW family protein -
N6G78_RS06925 (N6G78_06925) 1313529..1313693 + 165 WP_014479563.1 XkdX family protein -
N6G78_RS06930 (N6G78_06930) 1313740..1314579 + 840 WP_015383447.1 phage-like element PBSX protein XepA -
N6G78_RS06935 (N6G78_06935) 1314632..1314901 + 270 WP_015252265.1 hemolysin XhlA family protein -
N6G78_RS06940 (N6G78_06940) 1314914..1315177 + 264 WP_015483133.1 phage holin -
N6G78_RS06945 (N6G78_06945) 1315190..1316083 + 894 WP_015483134.1 N-acetylmuramoyl-L-alanine amidase -
N6G78_RS06950 (N6G78_06950) 1316121..1316258 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
N6G78_RS06955 (N6G78_06955) 1316344..1316514 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
N6G78_RS06960 (N6G78_06960) 1316514..1317260 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
N6G78_RS06965 (N6G78_06965) 1317370..1318371 - 1002 WP_003232642.1 inorganic phosphate transporter -
N6G78_RS06970 (N6G78_06970) 1318384..1319001 - 618 WP_003218470.1 DUF47 domain-containing protein -
N6G78_RS06975 (N6G78_06975) 1319277..1320593 - 1317 WP_015383450.1 serine/threonine exchanger -
N6G78_RS06980 (N6G78_06980) 1320982..1321932 + 951 WP_015383451.1 ring-cleaving dioxygenase -
N6G78_RS06985 (N6G78_06985) 1322034..1322141 + 108 Protein_1312 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1282326..1325660 43334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T259345 WP_003244695.1 NZ_CP104855:c1317260-1316514 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT259345 WP_003232646.1 NZ_CP104855:c1316514-1316344 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References