Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509770..510406 Replicon chromosome
Accession NZ_CP104855
Organism Bacillus subtilis strain TY-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag N6G78_RS02600 Protein ID WP_003156187.1
Coordinates 510056..510406 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag N6G78_RS02595 Protein ID WP_003225183.1
Coordinates 509770..510051 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6G78_RS02575 (N6G78_02575) 506129..506728 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
N6G78_RS02580 (N6G78_02580) 506823..507188 + 366 WP_015252768.1 holo-ACP synthase -
N6G78_RS02585 (N6G78_02585) 507354..508370 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
N6G78_RS02590 (N6G78_02590) 508485..509654 + 1170 WP_015382833.1 alanine racemase -
N6G78_RS02595 (N6G78_02595) 509770..510051 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
N6G78_RS02600 (N6G78_02600) 510056..510406 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N6G78_RS02605 (N6G78_02605) 510522..511346 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
N6G78_RS02610 (N6G78_02610) 511351..511716 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
N6G78_RS02615 (N6G78_02615) 511720..512121 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
N6G78_RS02620 (N6G78_02620) 512133..513140 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
N6G78_RS02625 (N6G78_02625) 513202..513531 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
N6G78_RS02630 (N6G78_02630) 513528..514010 + 483 WP_003234299.1 anti-sigma B factor RsbW -
N6G78_RS02635 (N6G78_02635) 513976..514764 + 789 WP_015482833.1 RNA polymerase sigma factor SigB -
N6G78_RS02640 (N6G78_02640) 514764..515363 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T259344 WP_003156187.1 NZ_CP104855:510056-510406 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT259344 WP_003225183.1 NZ_CP104855:509770-510051 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References