Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 84765..85408 Replicon plasmid pHN4E2-NDM5
Accession NZ_CP104852
Organism Escherichia coli strain GYX208DH4E-2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag N6H16_RS23985 Protein ID WP_001044768.1
Coordinates 84992..85408 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag N6H16_RS23980 Protein ID WP_001261287.1
Coordinates 84765..84995 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H16_RS23970 (79943) 79943..81076 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
N6H16_RS23975 (81339) 81339..84458 - 3120 WP_023909028.1 hypothetical protein -
N6H16_RS23980 (84765) 84765..84995 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N6H16_RS23985 (84992) 84992..85408 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
N6H16_RS23990 (85570) 85570..87708 - 2139 WP_000350635.1 AAA family ATPase -
N6H16_RS23995 (88173) 88173..89168 + 996 WP_000246636.1 hypothetical protein -
N6H16_RS24000 (89211) 89211..90104 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sul1 / qacE / aadA2 / dfrA12 / blaNDM-5 / aadA5 / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaOXA-1 / aac(6')-Ib-cr - 1..99713 99713
- flank IS/Tn - - 90241..90744 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T259343 WP_001044768.1 NZ_CP104852:84992-85408 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT259343 WP_001261287.1 NZ_CP104852:84765-84995 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References