Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 340259..341059 Replicon chromosome
Accession NZ_CP104851
Organism Escherichia coli strain GYX208DH4E-2

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag N6H16_RS01605 Protein ID WP_000342449.1
Coordinates 340532..341059 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag N6H16_RS01600 Protein ID WP_001277108.1
Coordinates 340259..340525 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H16_RS01580 (335916) 335916..336584 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
N6H16_RS01585 (336577) 336577..337635 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
N6H16_RS01590 (337880) 337880..338734 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
N6H16_RS01595 (339006) 339006..340109 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
N6H16_RS01600 (340259) 340259..340525 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
N6H16_RS01605 (340532) 340532..341059 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
N6H16_RS01610 (341056) 341056..341439 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
N6H16_RS01615 (341863) 341863..342972 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
N6H16_RS01620 (343020) 343020..343946 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
N6H16_RS01625 (343943) 343943..345220 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
N6H16_RS01630 (345217) 345217..345984 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T259320 WP_000342449.1 NZ_CP104851:340532-341059 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT259320 WP_001277108.1 NZ_CP104851:340259-340525 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References