Detailed information of TA system TA259295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1390819..1392508
• Replicon: chromosome
• Accession: NZ_CP104848
• Organism: Escherichia coli strain DFK.1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: N7218_RS06915
• Protein ID: WP_000365807.1
• Coordinates: 1391588..1392508 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: N7218_RS06910
• Protein ID: WP_001295795.1
• Coordinates: 1390819..1391601 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N7218_RS06905 (1387437)	1387437..1390535	-	3099	WP_000673070.1	type I restriction endonuclease	-
N7218_RS06910 (1390819)	1390819..1391601	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
N7218_RS06915 (1391588)	1391588..1392508	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
N7218_RS06920 (1392673)	1392673..1393449	-	777	WP_000171155.1	site-specific integrase	-
N7218_RS06925 (1393657)	1393657..1393992	-	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
N7218_RS06930 (1394219)	1394219..1394377	-	159	Protein_1361	integrase	-
N7218_RS06935 (1394426)	1394426..1394611	+	186	WP_000146240.1	hypothetical protein	-
N7218_RS06940 (1394826)	1394826..1395440	+	615	WP_001019923.1	YagU family protein	-
N7218_RS06945 (1395511)	1395511..1395677	+	167	Protein_1364	tail fiber assembly protein	-
N7218_RS06950 (1395689)	1395689..1396018	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
N7218_RS06955 (1396325)	1396325..1397035	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1384569..1432724	48155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T259295 WP_000365807.1 NZ_CP104848:1391588-1392508 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT259295 WP_001295795.1 NZ_CP104848:1390819-1391601 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0