Detailed information of TA system TA259260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1271498..1273187
• Replicon: chromosome
• Accession: NZ_CP104846
• Organism: Escherichia coli strain DFS.1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: N7219_RS06270
• Protein ID: WP_000365807.1
• Coordinates: 1272267..1273187 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: N7219_RS06265
• Protein ID: WP_001295795.1
• Coordinates: 1271498..1272280 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N7219_RS06260 (1268116)	1268116..1271214	-	3099	WP_000673070.1	type I restriction endonuclease	-
N7219_RS06265 (1271498)	1271498..1272280	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
N7219_RS06270 (1272267)	1272267..1273187	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
N7219_RS06275 (1273352)	1273352..1274128	-	777	WP_000171155.1	site-specific integrase	-
N7219_RS06280 (1274336)	1274336..1274671	-	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
N7219_RS06285 (1274898)	1274898..1275056	-	159	Protein_1232	integrase	-
N7219_RS06290 (1275105)	1275105..1275290	+	186	WP_000146240.1	hypothetical protein	-
N7219_RS06295 (1275505)	1275505..1276119	+	615	WP_001019923.1	YagU family protein	-
N7219_RS06300 (1276190)	1276190..1276356	+	167	Protein_1235	tail fiber assembly protein	-
N7219_RS06305 (1276368)	1276368..1276697	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
N7219_RS06310 (1277004)	1277004..1277714	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1265248..1313403	48155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T259260 WP_000365807.1 NZ_CP104846:1272267-1273187 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT259260 WP_001295795.1 NZ_CP104846:1271498-1272280 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0