Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 838793..839369 Replicon chromosome
Accession NZ_CP104846
Organism Escherichia coli strain DFS.1

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag N7219_RS04075 Protein ID WP_001295743.1
Coordinates 838793..839080 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag N7219_RS04080 Protein ID WP_000063148.1
Coordinates 839067..839369 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7219_RS04060 (834453) 834453..835757 - 1305 WP_000535012.1 restriction endonuclease subunit S -
N7219_RS04065 (835747) 835747..837366 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
N7219_RS04070 (837430) 837430..838596 - 1167 WP_000800831.1 restriction endonuclease -
N7219_RS04075 (838793) 838793..839080 + 288 WP_001295743.1 BrnT family toxin Toxin
N7219_RS04080 (839067) 839067..839369 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
N7219_RS04085 (839403) 839403..840359 - 957 WP_001295745.1 GTPase -
N7219_RS04090 (840370) 840370..840573 - 204 WP_000467859.1 YbdD/YjiX family protein -
N7219_RS04095 (840623) 840623..842773 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T259257 WP_001295743.1 NZ_CP104846:838793-839080 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT259257 WP_000063148.1 NZ_CP104846:839067-839369 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References