Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 20466..21264 Replicon plasmid unnamed1
Accession NZ_CP104844
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag N7217_RS24715 Protein ID WP_000072677.1
Coordinates 20466..20987 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag N7217_RS24720 Protein ID WP_001351987.1
Coordinates 20995..21264 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS24700 (N7217_24700) 19015..19338 + 324 WP_000064175.1 hypothetical protein -
N7217_RS24705 (N7217_24705) 19352..20044 + 693 WP_012640731.1 hypothetical protein -
N7217_RS24710 (N7217_24710) 20046..20297 + 252 WP_000901559.1 hypothetical protein -
N7217_RS24715 (N7217_24715) 20466..20987 - 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
N7217_RS24720 (N7217_24720) 20995..21264 - 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
N7217_RS24725 (N7217_24725) 21583..22251 + 669 WP_000161228.1 division plane positioning ATPase MipZ -
N7217_RS24730 (N7217_24730) 22257..22610 + 354 WP_160378290.1 hypothetical protein -
N7217_RS24735 (N7217_24735) 22671..23744 - 1074 WP_234288622.1 hypothetical protein -
N7217_RS24740 (N7217_24740) 24019..24759 + 741 WP_001717320.1 hypothetical protein -
N7217_RS24745 (N7217_24745) 24803..26143 + 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-55 - 1..112215 112215


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T259251 WP_000072677.1 NZ_CP104844:c20987-20466 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT259251 WP_001351987.1 NZ_CP104844:c21264-20995 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References