Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4517063..4517560 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N7217_RS21910 Protein ID WP_001173343.1
Coordinates 4517063..4517236 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N7217_RS21915 Protein ID WP_000398619.1
Coordinates 4517264..4517560 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS21900 (4513733) 4513733..4515190 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
N7217_RS21905 (4515427) 4515427..4516944 + 1518 WP_022646456.1 lysine--tRNA ligase -
N7217_RS21910 (4517063) 4517063..4517236 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N7217_RS21915 (4517264) 4517264..4517560 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N7217_RS21920 (4517789) 4517789..4518061 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N7217_RS21925 (4518073) 4518073..4518303 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
N7217_RS21930 (4518484) 4518484..4520115 + 1632 WP_022646455.1 sensor histidine kinase -
N7217_RS21935 (4520112) 4520112..4520831 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4481301..4517560 36259


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T259248 WP_001173343.1 NZ_CP104843:c4517236-4517063 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT259248 WP_000398619.1 NZ_CP104843:c4517560-4517264 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References