Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4244411..4244987 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N7217_RS20560 Protein ID WP_001297643.1
Coordinates 4244700..4244987 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag N7217_RS20555 Protein ID WP_000063156.1
Coordinates 4244411..4244713 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS20540 (4241007) 4241007..4243157 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N7217_RS20545 (4243207) 4243207..4243410 + 204 WP_000467859.1 YbdD/YjiX family protein -
N7217_RS20550 (4243421) 4243421..4244377 + 957 WP_001297640.1 GTPase -
N7217_RS20555 (4244411) 4244411..4244713 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
N7217_RS20560 (4244700) 4244700..4244987 - 288 WP_001297643.1 BrnT family toxin Toxin
N7217_RS20565 (4245186) 4245186..4245883 - 698 Protein_4026 IS1 family transposase -
N7217_RS20570 (4245940) 4245940..4246035 + 96 Protein_4027 dienelactone hydrolase family protein -
N7217_RS20575 (4246181) 4246181..4247413 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
N7217_RS20580 (4247454) 4247454..4248734 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T259245 WP_001297643.1 NZ_CP104843:c4244987-4244700 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT259245 WP_000063156.1 NZ_CP104843:c4244713-4244411 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References