Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3037857..3038641 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag N7217_RS14725 Protein ID WP_000613626.1
Coordinates 3038147..3038641 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag N7217_RS14720 Protein ID WP_024946541.1
Coordinates 3037857..3038150 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS14710 (3033018) 3033018..3033977 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
N7217_RS14715 (3034550) 3034550..3037723 + 3174 WP_022645526.1 ribonuclease E -
N7217_RS14720 (3037857) 3037857..3038150 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
N7217_RS14725 (3038147) 3038147..3038641 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
N7217_RS14730 (3038736) 3038736..3039689 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
N7217_RS14735 (3039701) 3039701..3041344 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
N7217_RS14740 (3041410) 3041410..3042351 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
N7217_RS14745 (3042351) 3042351..3043448 - 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T259239 WP_000613626.1 NZ_CP104843:3038147-3038641 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT259239 WP_024946541.1 NZ_CP104843:3037857-3038150 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References