Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 82759..83285 Replicon plasmid unnamed5
Accession NZ_CP104841
Organism Enterobacter cloacae strain 2022CK-00409

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag N7S94_RS26435 Protein ID WP_000323025.1
Coordinates 82759..83046 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag N7S94_RS26440 Protein ID WP_000534858.1
Coordinates 83046..83285 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7S94_RS26400 (N7S94_26395) 77900..78427 - 528 WP_007372155.1 hypothetical protein -
N7S94_RS26405 (N7S94_26400) 78507..79391 - 885 WP_007372154.1 hypothetical protein -
N7S94_RS26410 (N7S94_26405) 79591..79992 - 402 WP_020996135.1 hypothetical protein -
N7S94_RS26415 (N7S94_26410) 80083..80544 - 462 WP_009653863.1 hypothetical protein -
N7S94_RS26420 (N7S94_26415) 80928..81263 - 336 WP_008786801.1 PerC family transcriptional regulator -
N7S94_RS26425 (N7S94_26420) 81272..81589 - 318 WP_020996134.1 hypothetical protein -
N7S94_RS26430 (N7S94_26425) 81699..82706 - 1008 WP_261659470.1 phosphoadenosine phosphosulfate reductase family protein -
N7S94_RS26435 (N7S94_26430) 82759..83046 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
N7S94_RS26440 (N7S94_26435) 83046..83285 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
N7S94_RS26445 (N7S94_26440) 83310..83414 + 105 Protein_98 hypothetical protein -
N7S94_RS26450 (N7S94_26445) 83548..84471 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
N7S94_RS26455 (N7S94_26450) 84671..85243 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
N7S94_RS26460 (N7S94_26455) 85886..87226 - 1341 WP_000589001.1 ISNCY family transposase -
N7S94_RS26465 (N7S94_26460) 87378..87701 - 324 WP_020996132.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..318750 318750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T259225 WP_000323025.1 NZ_CP104841:c83046-82759 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT259225 WP_000534858.1 NZ_CP104841:c83285-83046 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References