Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 167828..168567 Replicon plasmid unnamed2
Accession NZ_CP104838
Organism Enterobacter cloacae strain 2022CK-00409

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag N7S94_RS25560 Protein ID WP_013087172.1
Coordinates 168082..168567 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag N7S94_RS25555 Protein ID WP_012540086.1
Coordinates 167828..168094 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7S94_RS25525 (N7S94_25520) 163058..163594 - 537 WP_178328939.1 GNAT family N-acetyltransferase -
N7S94_RS25530 (N7S94_25525) 163719..165470 - 1752 WP_178328940.1 arsenical pump-driving ATPase -
N7S94_RS25535 (N7S94_25530) 165497..165859 - 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
N7S94_RS25540 (N7S94_25535) 165935..166480 - 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -
N7S94_RS25545 (N7S94_25540) 166489..167202 - 714 WP_178328938.1 arsenical resistance protein ArsH -
N7S94_RS25550 (N7S94_25545) 167204..167527 - 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
N7S94_RS25555 (N7S94_25550) 167828..168094 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
N7S94_RS25560 (N7S94_25555) 168082..168567 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
N7S94_RS25565 (N7S94_25560) 168942..169639 + 698 WP_261659429.1 IS1 family transposase -
N7S94_RS25570 (N7S94_25565) 169853..170836 + 984 WP_213382218.1 tyrosine-type recombinase/integrase -
N7S94_RS25575 (N7S94_25570) 171204..172823 + 1620 WP_023332837.1 phosphoethanolamine--lipid A transferase MCR-10.1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 - 1..189847 189847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T259224 WP_013087172.1 NZ_CP104838:168082-168567 [Enterobacter cloacae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT259224 WP_012540086.1 NZ_CP104838:167828-168094 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References