Detailed information of TA system TA025922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3042980..3043550
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: BCGMEX_RS14390
• Protein ID: WP_003414166.1
• Coordinates: 3042980..3043336 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: BCGMEX_RS14395
• Protein ID: WP_003901465.1
• Coordinates: 3043320..3043550 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS14370	3038432..3040120	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
BCGMEX_RS14375	3040124..3040450	-	327	WP_003414157.1	hypothetical protein	-
BCGMEX_RS14380	3040623..3041210	+	588	WP_003914429.1	DUF3558 family protein	-
BCGMEX_RS14385	3041229..3042878	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
BCGMEX_RS14390	3042980..3043336	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
BCGMEX_RS14395	3043320..3043550	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
BCGMEX_RS14400	3043593..3044636	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
BCGMEX_RS21880	3044827..3045102	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
BCGMEX_RS21885	3045278..3045532	-	255	WP_003917684.1	hypothetical protein	-
BCGMEX_RS14415	3045680..3046084	+	405	WP_003414181.1	hypothetical protein	-
BCGMEX_RS14420	3046081..3046272	+	192	WP_003414184.1	hypothetical protein	-
BCGMEX_RS14425	3046336..3047625	+	1290	Protein_2815	transposase family protein	-
BCGMEX_RS14430	3047859..3048116	+	258	WP_003899489.1	hypothetical protein	-
BCGMEX_RS14435	3048221..3048532	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T25922 WP_003414166.1 NC_016804:c3043336-3042980 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T25922 NC_016804:c3043336-3042980 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT25922 WP_003901465.1 NC_016804:c3043550-3043320 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT25922 NC_016804:c3043550-3043320 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3