Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2712850..2713454 Replicon chromosome
Accession NZ_CP104836
Organism Enterobacter cloacae strain 2022CK-00409

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag N7S94_RS13350 Protein ID WP_071843252.1
Coordinates 2713269..2713454 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Z1F6W0
Locus tag N7S94_RS13345 Protein ID WP_013094943.1
Coordinates 2712850..2713254 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7S94_RS13330 (N7S94_13325) 2708387..2709205 - 819 WP_161657006.1 helix-turn-helix domain-containing protein -
N7S94_RS13335 (N7S94_13330) 2709430..2710830 + 1401 WP_020686524.1 MFS transporter -
N7S94_RS13340 (N7S94_13335) 2710841..2712796 + 1956 WP_261658677.1 glycoside hydrolase family 127 protein -
N7S94_RS13345 (N7S94_13340) 2712850..2713254 - 405 WP_013094943.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N7S94_RS13350 (N7S94_13345) 2713269..2713454 - 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
N7S94_RS13355 (N7S94_13350) 2713665..2714258 - 594 Protein_2614 MipA/OmpV family protein -
N7S94_RS13360 (N7S94_13355) 2714405..2715385 - 981 WP_211944394.1 IS5-like element ISKpn26 family transposase -
N7S94_RS13365 (N7S94_13360) 2715456..2715794 + 339 Protein_2616 LysR substrate-binding domain-containing protein -
N7S94_RS13370 (N7S94_13365) 2715791..2717329 - 1539 WP_020686520.1 aldehyde dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2714405..2715385 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T259219 WP_071843252.1 NZ_CP104836:c2713454-2713269 [Enterobacter cloacae]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14915.74 Da        Isoelectric Point: 4.3036

>AT259219 WP_013094943.1 NZ_CP104836:c2713254-2712850 [Enterobacter cloacae]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVNEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F6W0

References