Detailed information of TA system TA025921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3003397..3004084
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: BCGMEX_RS14170
• Protein ID: WP_003414064.1
• Coordinates: 3003689..3004084 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BCGMEX_RS14165
• Protein ID: WP_003414061.1
• Coordinates: 3003397..3003663 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS14140	2999036..2999938	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BCGMEX_RS14145	3000007..3000759	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BCGMEX_RS14150	3001003..3001278	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BCGMEX_RS14155	3001275..3002897	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
BCGMEX_RS14160	3002984..3003400	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
BCGMEX_RS14165	3003397..3003663	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGMEX_RS14170	3003689..3004084	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGMEX_RS14175	3004081..3004350	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BCGMEX_RS14180	3004360..3005454	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BCGMEX_RS14185	3005451..3005870	-	420	Protein_2768	winged helix-turn-helix domain-containing protein	-
BCGMEX_RS14190	3005944..3006423	-	480	WP_003414073.1	dihydrofolate reductase	-
BCGMEX_RS14195	3006494..3007294	-	801	WP_003911953.1	thymidylate synthase	-
BCGMEX_RS14200	3007450..3008187	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T25921 WP_003414064.1 NC_016804:c3004084-3003689 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T25921 NC_016804:c3004084-3003689 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT25921 WP_003414061.1 NC_016804:c3003663-3003397 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT25921 NC_016804:c3003663-3003397 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64