Detailed information of TA system TA259200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4857298..4858979
• Replicon: chromosome
• Accession: NZ_CP104796
• Organism: Klebsiella pneumoniae strain GX34

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: N7T97_RS24490
• Protein ID: WP_004146663.1
• Coordinates: 4857298..4858215 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: N7T97_RS24495
• Protein ID: WP_004177809.1
• Coordinates: 4858221..4858979 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N7T97_RS24470 (4852604)	4852604..4854124	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
N7T97_RS24475 (4854121)	4854121..4855119	+	999	WP_012737175.1	ribose ABC transporter permease	-
N7T97_RS24480 (4855150)	4855150..4856091	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
N7T97_RS24485 (4856166)	4856166..4857173	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
N7T97_RS24490 (4857298)	4857298..4858215	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
N7T97_RS24495 (4858221)	4858221..4858979	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
N7T97_RS24500 (4859065)	4859065..4859793	-	729	WP_004177810.1	response regulator transcription factor	-
N7T97_RS24505 (4860066)	4860066..4862213	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
N7T97_RS24510 (4862443)	4862443..4863129	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T259200 WP_004146663.1 NZ_CP104796:c4858215-4857298 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT259200 WP_004177809.1 NZ_CP104796:c4858979-4858221 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05