Detailed information of TA system TA259180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 58848..59473
• Replicon: plasmid pHNSHP24-2
• Accession: NZ_CP104790
• Organism: Escherichia coli strain SHP24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: N6H17_RS25465
• Protein ID: WP_096973166.1
• Coordinates: 58848..59246 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: N6H17_RS25470
• Protein ID: WP_000450530.1
• Coordinates: 59246..59473 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N6H17_RS25430 (53893)	53893..54864	+	972	WP_000817026.1	ParB/RepB/Spo0J family plasmid partition protein	-
N6H17_RS25435 (55377)	55377..55628	+	252	WP_001132891.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N6H17_RS25440 (55625)	55625..55912	+	288	WP_000222773.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N6H17_RS25445 (56048)	56048..56442	+	395	Protein_54	helix-turn-helix domain-containing protein	-
N6H17_RS25450 (56631)	56631..57679	-	1049	Protein_55	transposase	-
N6H17_RS25455 (57695)	57695..58368	-	674	Protein_56	transposase	-
N6H17_RS25460 (58441)	58441..58839	+	399	Protein_57	conjugal transfer protein TraD	-
N6H17_RS25465 (58848)	58848..59246	-	399	WP_096973166.1	type II toxin-antitoxin system VapC family toxin	Toxin
N6H17_RS25470 (59246)	59246..59473	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic	1..186621	186621
	-	inside	IScluster/Tn	-	-	46301..58368	12067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14899.22 Da
• Isoelectric Point: 8.5264

>T259180 WP_096973166.1 NZ_CP104790:c59246-58848 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVVGLRIEDWS

Nucleotide

• Length: 399 bp

>T259180 NZ_CP104790:c59246-58848 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGTCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT259180 WP_000450530.1 NZ_CP104790:c59473-59246 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT259180 NZ_CP104790:c59473-59246 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2