Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2813544..2814181 Replicon chromosome
Accession NC_016804
Organism Mycobacterium bovis BCG str. Mexico

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BCGMEX_RS13170 Protein ID WP_003413180.1
Coordinates 2813544..2813939 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BCGMEX_RS13175 Protein ID WP_003413183.1
Coordinates 2813936..2814181 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCGMEX_RS13130 2809629..2810288 + 660 WP_003900846.1 LppA family lipoprotein -
BCGMEX_RS13135 2810285..2810944 + 660 WP_003900847.1 hypothetical protein -
BCGMEX_RS13140 2810941..2811603 + 663 WP_003900848.1 hypothetical protein -
BCGMEX_RS13145 2811600..2811878 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BCGMEX_RS13150 2811971..2812384 + 414 WP_003413164.1 PIN domain nuclease -
BCGMEX_RS13155 2812423..2812680 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BCGMEX_RS13160 2812677..2813054 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BCGMEX_RS13165 2813070..2813444 - 375 WP_003413177.1 hypothetical protein -
BCGMEX_RS13170 2813544..2813939 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BCGMEX_RS13175 2813936..2814181 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BCGMEX_RS13180 2814592..2815011 - 420 WP_003413190.1 A24 family peptidase -
BCGMEX_RS13185 2815023..2815850 - 828 WP_010950736.1 shikimate dehydrogenase -
BCGMEX_RS13190 2815828..2817081 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BCGMEX_RS13195 2817074..2817586 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T25918 WP_003413180.1 NC_016804:c2813939-2813544 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T25918 NC_016804:c2813939-2813544 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT25918 WP_003413183.1 NC_016804:c2814181-2813936 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT25918 NC_016804:c2814181-2813936 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References