Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 140728..142351 Replicon plasmid pHNSHP24-1
Accession NZ_CP104789
Organism Escherichia coli strain SHP24

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag N6H17_RS24480 Protein ID WP_000088046.1
Coordinates 140728..142053 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag N6H17_RS24485 Protein ID WP_024136327.1
Coordinates 142058..142351 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H17_RS24450 (N6H17_24460) 137398..137697 - 300 WP_001572440.1 hypothetical protein -
N6H17_RS24455 (N6H17_24465) 137752..137988 - 237 Protein_132 type I restriction-modification system subunit M N-terminal domain-containing protein -
N6H17_RS24460 (N6H17_24470) 138442..138867 - 426 WP_001043843.1 hypothetical protein -
N6H17_RS24465 (N6H17_24475) 139121..139936 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
N6H17_RS24470 (N6H17_24480) 139949..140359 - 411 WP_000985911.1 hypothetical protein -
N6H17_RS24475 (N6H17_24485) 140461..140667 - 207 WP_000134171.1 hypothetical protein -
N6H17_RS24480 (N6H17_24490) 140728..142053 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
N6H17_RS24485 (N6H17_24495) 142058..142351 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
N6H17_RS24490 (N6H17_24500) 142955..143347 - 393 WP_000341066.1 hypothetical protein -
N6H17_RS24495 (N6H17_24505) 143720..144061 + 342 WP_001151575.1 hypothetical protein -
N6H17_RS24500 (N6H17_24510) 144153..144344 + 192 WP_000398480.1 hypothetical protein -
N6H17_RS24505 (N6H17_24515) 144394..144675 + 282 WP_000077926.1 hypothetical protein -
N6H17_RS24510 (N6H17_24520) 145016..145147 - 132 WP_000374059.1 hypothetical protein -
N6H17_RS24515 (N6H17_24525) 145238..146479 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aph(3')-Ia / dfrA12 / oqxA / oqxB - 1..259017 259017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T259177 WP_000088046.1 NZ_CP104789:c142053-140728 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT259177 WP_024136327.1 NZ_CP104789:c142351-142058 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References