Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4788429..4789005 Replicon chromosome
Accession NZ_CP104788
Organism Escherichia coli strain SHP24

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N6H17_RS23465 Protein ID WP_001297643.1
Coordinates 4788429..4788716 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag N6H17_RS23470 Protein ID WP_000063156.1
Coordinates 4788703..4789005 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H17_RS23445 (4784682) 4784682..4785962 - 1281 WP_001037380.1 DUF445 domain-containing protein -
N6H17_RS23450 (4786003) 4786003..4787235 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
N6H17_RS23455 (4787381) 4787381..4787476 - 96 Protein_4567 dienelactone hydrolase family protein -
N6H17_RS23460 (4787533) 4787533..4788230 + 698 Protein_4568 IS1 family transposase -
N6H17_RS23465 (4788429) 4788429..4788716 + 288 WP_001297643.1 BrnT family toxin Toxin
N6H17_RS23470 (4788703) 4788703..4789005 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
N6H17_RS23475 (4789039) 4789039..4789995 - 957 WP_001297640.1 GTPase -
N6H17_RS23480 (4790006) 4790006..4790209 - 204 WP_000467859.1 YbdD/YjiX family protein -
N6H17_RS23485 (4790259) 4790259..4792409 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T259176 WP_001297643.1 NZ_CP104788:4788429-4788716 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT259176 WP_000063156.1 NZ_CP104788:4788703-4789005 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References