Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4571606..4572103 Replicon chromosome
Accession NZ_CP104788
Organism Escherichia coli strain SHP24

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N6H17_RS22335 Protein ID WP_001173343.1
Coordinates 4571930..4572103 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N6H17_RS22330 Protein ID WP_000398619.1
Coordinates 4571606..4571902 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H17_RS22310 (4568337) 4568337..4569056 - 720 WP_000611288.1 two-component system response regulator DcuR -
N6H17_RS22315 (4569053) 4569053..4570684 - 1632 WP_001216477.1 sensor histidine kinase -
N6H17_RS22320 (4570865) 4570865..4571095 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N6H17_RS22325 (4571107) 4571107..4571379 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
N6H17_RS22330 (4571606) 4571606..4571902 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N6H17_RS22335 (4571930) 4571930..4572103 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N6H17_RS22340 (4572222) 4572222..4573739 - 1518 WP_001295074.1 lysine--tRNA ligase -
N6H17_RS22345 (4573976) 4573976..4575433 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T259174 WP_001173343.1 NZ_CP104788:4571930-4572103 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT259174 WP_000398619.1 NZ_CP104788:4571606-4571902 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References