Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5323160..5323785 Replicon chromosome
Accession NZ_CP104766
Organism Klebsiella pneumoniae strain IMT44613

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N7Q99_RS25805 Protein ID WP_002882817.1
Coordinates 5323160..5323543 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N7Q99_RS25810 Protein ID WP_004150355.1
Coordinates 5323543..5323785 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7Q99_RS25790 (5320526) 5320526..5321428 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N7Q99_RS25795 (5321425) 5321425..5322060 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N7Q99_RS25800 (5322057) 5322057..5322986 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N7Q99_RS25805 (5323160) 5323160..5323543 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N7Q99_RS25810 (5323543) 5323543..5323785 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N7Q99_RS25815 (5323990) 5323990..5324907 + 918 WP_009484979.1 alpha/beta hydrolase -
N7Q99_RS25820 (5324921) 5324921..5325862 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N7Q99_RS25825 (5325907) 5325907..5326344 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N7Q99_RS25830 (5326341) 5326341..5327201 - 861 WP_004146232.1 virulence factor BrkB family protein -
N7Q99_RS25835 (5327195) 5327195..5327794 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T259152 WP_002882817.1 NZ_CP104766:c5323543-5323160 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT259152 WP_004150355.1 NZ_CP104766:c5323785-5323543 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References