Detailed information of TA system TA025915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2797428..2798068
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BCGMEX_RS13060
• Protein ID: WP_003412970.1
• Coordinates: 2797428..2797847 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BCGMEX_RS13065
• Protein ID: WP_003412975.1
• Coordinates: 2797844..2798068 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS13035	2793013..2793735	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BCGMEX_RS13040	2794252..2794479	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
BCGMEX_RS13045	2794476..2794877	+	402	WP_003412963.1	PIN domain-containing protein	-
BCGMEX_RS13050	2794912..2795832	-	921	WP_011799273.1	restriction endonuclease	-
BCGMEX_RS22140	2796173..2796418	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BCGMEX_RS13055	2796477..2797427	+	951	WP_003911916.1	Lsr2 family protein	-
BCGMEX_RS13060	2797428..2797847	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGMEX_RS13065	2797844..2798068	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BCGMEX_RS13070	2798099..2800942	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BCGMEX_RS13075	2801014..2801415	-	402	WP_003412981.1	hypothetical protein	-
BCGMEX_RS13080	2801415..2801885	-	471	WP_003412985.1	transcription antitermination factor NusB	-
BCGMEX_RS13085	2801888..2802451	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T25915 WP_003412970.1 NC_016804:c2797847-2797428 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T25915 NC_016804:c2797847-2797428 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT25915 WP_003412975.1 NC_016804:c2798068-2797844 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT25915 NC_016804:c2798068-2797844 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09