Detailed information of TA system TA259137

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 238811..240561
• Replicon: plasmid p1_Er676
• Accession: NZ_CP104765
• Organism: Enterococcus raffinosus strain Er676

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: N7K39_RS17265
• Protein ID: WP_028020616.1
• Coordinates: 239677..240561 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: A0A1L8WJA7
• Locus tag: N7K39_RS17260
• Protein ID: WP_028020615.1
• Coordinates: 238811..239680 (+)
• Length: 290 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N7K39_RS17235 (N7K39_17235)	234985..235650	+	666	WP_028020609.1	type II CAAX endopeptidase family protein	-
N7K39_RS17240 (N7K39_17240)	235951..236385	-	435	WP_028020610.1	hypothetical protein	-
N7K39_RS17245 (N7K39_17245)	236868..237169	-	302	Protein_231	type II toxin-antitoxin system PemK/MazF family toxin	-
N7K39_RS17250 (N7K39_17250)	237201..237407	-	207	WP_028020612.1	hypothetical protein	-
N7K39_RS17255 (N7K39_17255)	238331..238669	-	339	WP_028020614.1	hypothetical protein	-
N7K39_RS17260 (N7K39_17260)	238811..239680	+	870	WP_028020615.1	hypothetical protein	Antitoxin
N7K39_RS17265 (N7K39_17265)	239677..240561	+	885	WP_028020616.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
N7K39_RS17270 (N7K39_17270)	241608..241919	+	312	WP_028020617.1	heavy metal-binding domain-containing protein	-
N7K39_RS17275 (N7K39_17275)	241934..242548	+	615	WP_028020618.1	CPBP family intramembrane metalloprotease	-
N7K39_RS17280 (N7K39_17280)	242700..243398	-	699	WP_028020619.1	hypothetical protein	-
N7K39_RS17285 (N7K39_17285)	244029..245144	+	1116	WP_028020620.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bsh	1..1144253	1144253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (31-273)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 34333.01 Da
• Isoelectric Point: 4.8781

>T259137 WP_028020616.1 NZ_CP104765:239677-240561 [Enterococcus raffinosus]
MNSDRLRDLIKAKKGSIASAEELRIRYATERFLLRVQASPYKDKLILKGGFLLGTIYKIENRTTKDLDALVKDIAADRES
VKSMLEEIITIDLNDDVSFELGNFSDSQHERKYTGFRAKLTMQHLNSTAKIAFDLDLGVGDVVTPEPKVLTFNLTFKEQK
GENERITMYSYPIETVLAEKVETIFALGIQNTRMKDFYDIHLLLNDPNKPSLENLYEAFNKTWIFRHDQIEDADFEDWLY
LVNELKDNQNFKKGLWQNYITNREYAQSLSWGEIIDELIEYIIALQKYRNETKE

Nucleotide

• Length: 885 bp

Antitoxin

Protein

• Length: 290 a.a.
• Molecular weight: 33188.75 Da
• Isoelectric Point: 6.3972

>AT259137 WP_028020615.1 NZ_CP104765:238811-239680 [Enterococcus raffinosus]
MTNRSNLDHSEEINRLLDTVKEMNPEALKDVQEFLEQLTLGDRLSRYIDFDELPAEDTDEFTFLKLVVDSKGIIIKKRLG
TVGLTHYKAILFLSKYGFQSMYGGSVYISPKNALDGVYIFQQQYSKAVISHETALYFHDLTDVIPKTTIMSLPKTYNLNQ
LFVWDGESPHLTVSKAPSTFHNKVRVIVHYSGNDSIDIVRNNEIKSSEIETFETTFGNQVRVTTPERTIADILKTSSYTE
AEVMFDAVKRYMNNPQSKELRLRRIADEQGVKKRLDECLTLLRSRGEIK

Nucleotide

• Length: 870 bp

Structures

Antitoxin

• AlphaFold DB: A0A1L8WJA7