Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 656273..656856 Replicon plasmid p1_ATCC49464
Accession NZ_CP104763
Organism Enterococcus raffinosus strain ATCC 49464

Toxin (Protein)


Gene name mazF Uniprot ID R2S2E9
Locus tag N7K38_RS19475 Protein ID WP_010743953.1
Coordinates 656512..656856 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R2RFE4
Locus tag N7K38_RS19470 Protein ID WP_010743954.1
Coordinates 656273..656518 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7K38_RS19440 (N7K38_19440) 651494..652798 + 1305 WP_010743959.1 glycosyltransferase family 2 protein -
N7K38_RS19445 (N7K38_19445) 652925..654010 + 1086 WP_141741229.1 UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) -
N7K38_RS19450 (N7K38_19450) 654098..654190 - 93 WP_111945260.1 type I toxin-antitoxin system Fst family toxin -
N7K38_RS19455 (N7K38_19455) 654272..654889 - 618 WP_010743957.1 DUF2922 family protein -
N7K38_RS19460 (N7K38_19460) 654935..655319 - 385 Protein_597 hypothetical protein -
N7K38_RS19465 (N7K38_19465) 655728..655856 + 129 WP_010743955.1 hypothetical protein -
N7K38_RS19470 (N7K38_19470) 656273..656518 + 246 WP_010743954.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
N7K38_RS19475 (N7K38_19475) 656512..656856 + 345 WP_010743953.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N7K38_RS19480 (N7K38_19480) 657304..657969 + 666 WP_035015986.1 helix-turn-helix domain-containing protein -
N7K38_RS19485 (N7K38_19485) 658463..659935 + 1473 WP_010743951.1 helix-turn-helix domain-containing protein -
N7K38_RS19490 (N7K38_19490) 659952..660731 + 780 WP_010743950.1 AP2 domain-containing protein -
N7K38_RS19495 (N7K38_19495) 660831..661244 + 414 WP_010743949.1 Ohr family peroxiredoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bsh 1..1021776 1021776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin

SpoVT-AbrB domain

(8-46)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13217.40 Da        Isoelectric Point: 9.2981

>T259134 WP_010743953.1 NZ_CP104763:656512-656856 [Enterococcus raffinosus]
MVRMIKQGDIVKMNLDPKQGHEQKGYRPYICLSYKGVAMNSRIAIFAPISNTQRNYPLYVPVSAECKTTGKVLLDQLVAI
DFYHRDYSFVETVPDDFINDLLKKVKVIFQRDKE

Download         Length: 345 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9154.35 Da        Isoelectric Point: 3.9490

>AT259134 WP_010743954.1 NZ_CP104763:656273-656518 [Enterococcus raffinosus]
MMQIPIGKWGNSNAIRIPVDLLNAIGAKQGDKLNVEIENGKLILGKNVDEELTFEQLFEGYDEEPFDSDLVMFEPTGNEK
W

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R2S2E9

Antitoxin

Source ID Structure
AlphaFold DB R2RFE4

References