Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4130845..4131421 Replicon chromosome
Accession NZ_CP104721
Organism Escherichia coli strain ECO3183

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N5O92_RS19805 Protein ID WP_001297643.1
Coordinates 4131134..4131421 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag N5O92_RS19800 Protein ID WP_000063160.1
Coordinates 4130845..4131147 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O92_RS19785 (4127441) 4127441..4129591 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N5O92_RS19790 (4129641) 4129641..4129844 + 204 WP_000467859.1 YbdD/YjiX family protein -
N5O92_RS19795 (4129855) 4129855..4130811 + 957 WP_001297640.1 GTPase -
N5O92_RS19800 (4130845) 4130845..4131147 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
N5O92_RS19805 (4131134) 4131134..4131421 - 288 WP_001297643.1 BrnT family toxin Toxin
N5O92_RS19810 (4131620) 4131620..4132316 - 697 Protein_3873 IS1 family transposase -
N5O92_RS19815 (4132631) 4132631..4133863 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
N5O92_RS19820 (4133904) 4133904..4135184 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4131620..4131829 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T259051 WP_001297643.1 NZ_CP104721:c4131421-4131134 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT259051 WP_000063160.1 NZ_CP104721:c4131147-4130845 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References