Detailed information of TA system TA025904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2176169..2176872
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BCGMEX_RS10055
• Protein ID: WP_003409778.1
• Coordinates: 2176169..2176498 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BCGMEX_RS20940
• Protein ID: WP_003409780.1
• Coordinates: 2176495..2176872 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS10035	2172552..2173622	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BCGMEX_RS10040	2173619..2174134	+	516	WP_003409718.1	flavin reductase family protein	-
BCGMEX_RS10045	2174131..2175192	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BCGMEX_RS10050	2175189..2175959	+	771	WP_003409775.1	SDR family oxidoreductase	-
BCGMEX_RS10055	2176169..2176498	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCGMEX_RS20940	2176495..2176872	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BCGMEX_RS10065	2176869..2177459	-	591	WP_003910477.1	SEC-C domain-containing protein	-
BCGMEX_RS10070	2177514..2178878	+	1365	WP_011799233.1	HNH endonuclease	-
BCGMEX_RS10075	2179033..2179485	-	453	WP_010950636.1	lipoprotein	-
BCGMEX_RS10080	2179549..2179950	+	402	WP_003409869.1	hypothetical protein	-
BCGMEX_RS10085	2179943..2180125	-	183	WP_003409870.1	hypothetical protein	-
BCGMEX_RS10090	2180239..2180589	-	351	WP_003409871.1	hypothetical protein	-
BCGMEX_RS10095	2180600..2181502	-	903	WP_003409874.1	hypothetical protein	-
BCGMEX_RS10100	2181523..2181714	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T25904 WP_003409778.1 NC_016804:c2176498-2176169 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T25904 NC_016804:c2176498-2176169 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT25904 WP_003409780.1 NC_016804:c2176872-2176495 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT25904 NC_016804:c2176872-2176495 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8