259001

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 32852..33762 Replicon plasmid unnamed2
Accession NZ_CP104683
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag N5922_RS25325 Protein ID WP_004026354.1
Coordinates 32852..33322 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag N5922_RS25330 Protein ID WP_004181895.1
Coordinates 33319..33762 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5922_RS25285 (N5922_25280) 28196..28900 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
N5922_RS25290 (N5922_25285) 29284..29775 + 492 WP_004206662.1 hypothetical protein -
N5922_RS25295 (N5922_25290) 29836..30039 + 204 WP_004213596.1 HHA domain-containing protein -
N5922_RS25300 (N5922_25295) 30053..30274 + 222 WP_016237028.1 hypothetical protein -
N5922_RS25305 (N5922_25300) 30296..31135 + 840 Protein_36 IS5 family transposase -
N5922_RS25310 (N5922_25305) 31135..31440 + 306 Protein_37 integrase core domain-containing protein -
N5922_RS25315 (N5922_25310) 31391..31783 - 393 Protein_38 transposase -
N5922_RS25320 (N5922_25315) 32482..32742 + 261 WP_004026352.1 hypothetical protein -
N5922_RS25325 (N5922_25320) 32852..33322 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
N5922_RS25330 (N5922_25325) 33319..33762 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
N5922_RS25335 (N5922_25330) 33863..35134 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
N5922_RS25340 (N5922_25335) 35134..35502 - 369 WP_042014650.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
N5922_RS25345 (N5922_25340) 35595..36575 - 981 WP_103284490.1 IS5-like element ISKpn26 family transposase -
N5922_RS25350 (N5922_25345) 36601..37563 + 963 WP_261588837.1 IS5 family transposase -
N5922_RS25355 (N5922_25350) 37789..38486 + 698 WP_103215986.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..122304 122304
- inside IScluster/Tn - - 18717..42312 23595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T259001 WP_004026354.1 NZ_CP104683:c33322-32852 [Escherichia coli]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT259001 WP_004181895.1 NZ_CP104683:c33762-33319 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References