Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3588616..3589234 Replicon chromosome
Accession NZ_CP104681
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag N5922_RS17590 Protein ID WP_001291435.1
Coordinates 3589016..3589234 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag N5922_RS17585 Protein ID WP_000344800.1
Coordinates 3588616..3588990 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5922_RS17575 (3583705) 3583705..3584898 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
N5922_RS17580 (3584921) 3584921..3588070 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
N5922_RS17585 (3588616) 3588616..3588990 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
N5922_RS17590 (3589016) 3589016..3589234 + 219 WP_001291435.1 HHA domain-containing protein Toxin
N5922_RS17595 (3589406) 3589406..3589957 + 552 WP_000102564.1 maltose O-acetyltransferase -
N5922_RS17600 (3590073) 3590073..3590543 + 471 WP_000136192.1 YlaC family protein -
N5922_RS17605 (3590707) 3590707..3592257 + 1551 WP_016241944.1 cyclic-guanylate-specific phosphodiesterase PdeB -
N5922_RS17610 (3592299) 3592299..3592652 - 354 WP_000878140.1 DUF1428 domain-containing protein -
N5922_RS17620 (3593031) 3593031..3593342 + 312 WP_000409911.1 MGMT family protein -
N5922_RS17625 (3593373) 3593373..3593945 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T258993 WP_001291435.1 NZ_CP104681:3589016-3589234 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT258993 WP_000344800.1 NZ_CP104681:3588616-3588990 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References