Detailed information of TA system TA025898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1535183..1536233
• Replicon: chromosome
• Accession: NC_016804
• Organism: Mycobacterium bovis BCG str. Mexico

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: BCGMEX_RS07240
• Protein ID: WP_003407164.1
• Coordinates: 1535183..1536004 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: BCGMEX_RS07245
• Protein ID: WP_003407167.1
• Coordinates: 1535997..1536233 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGMEX_RS07220	1530741..1531403	-	663	WP_010950531.1	hypothetical protein	-
BCGMEX_RS07225	1531400..1532185	-	786	WP_003407157.1	membrane protein	-
BCGMEX_RS07230	1532476..1534437	-	1962	WP_010950533.1	sigma-F factor regulator	-
BCGMEX_RS07235	1534576..1534962	-	387	WP_003407162.1	anti-sigma-F factor antagonist RsfA	-
BCGMEX_RS07240	1535183..1536004	+	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
BCGMEX_RS07245	1535997..1536233	+	237	WP_003407167.1	hypothetical protein	Antitoxin
BCGMEX_RS07250	1536305..1537510	-	1206	WP_003918234.1	serine hydrolase	-
BCGMEX_RS07255	1537813..1538598	+	786	WP_003407180.1	lipoprotein LprF	-
BCGMEX_RS07260	1538794..1540264	+	1471	Protein_1410	fatty acid desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T25898 WP_003407164.1 NC_016804:1535183-1536004 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T25898 NC_016804:1535183-1536004 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT25898 WP_003407167.1 NC_016804:1535997-1536233 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT25898 NC_016804:1535997-1536233 [Mycobacterium tuberculosis variant bovis BCG str. Mexico]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: G0TIH2

Antitoxin

• AlphaFold DB: A0A7U4BTV8