Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5342618..5343243 Replicon chromosome
Accession NZ_CP104678
Organism Klebsiella pneumoniae strain 2021CK-00608

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N5933_RS26025 Protein ID WP_002882817.1
Coordinates 5342618..5343001 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N5933_RS26030 Protein ID WP_004150355.1
Coordinates 5343001..5343243 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5933_RS26010 (5339984) 5339984..5340886 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N5933_RS26015 (5340883) 5340883..5341518 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N5933_RS26020 (5341515) 5341515..5342444 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N5933_RS26025 (5342618) 5342618..5343001 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N5933_RS26030 (5343001) 5343001..5343243 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N5933_RS26035 (5343448) 5343448..5344365 + 918 WP_004181612.1 alpha/beta hydrolase -
N5933_RS26040 (5344379) 5344379..5345320 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N5933_RS26045 (5345365) 5345365..5345802 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N5933_RS26050 (5345799) 5345799..5346659 - 861 WP_004146232.1 virulence factor BrkB family protein -
N5933_RS26055 (5346653) 5346653..5347252 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T258974 WP_002882817.1 NZ_CP104678:c5343001-5342618 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT258974 WP_004150355.1 NZ_CP104678:c5343243-5343001 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References