Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3331365..3332070 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name relE Uniprot ID S1F406
Locus tag N5914_RS16030 Protein ID WP_000539521.1
Coordinates 3331365..3331751 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag N5914_RS16035 Protein ID WP_001280945.1
Coordinates 3331741..3332070 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS16010 (3327369) 3327369..3327995 + 627 WP_001295292.1 glutathione S-transferase GstB -
N5914_RS16015 (3327992) 3327992..3329107 - 1116 WP_000554964.1 aldose sugar dehydrogenase YliI -
N5914_RS16020 (3329218) 3329218..3329601 - 384 WP_000497137.1 biofilm formation regulator BssR -
N5914_RS16025 (3329814) 3329814..3331139 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
N5914_RS16030 (3331365) 3331365..3331751 + 387 WP_000539521.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5914_RS16035 (3331741) 3331741..3332070 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
N5914_RS16040 (3332140) 3332140..3333468 - 1329 WP_022645414.1 GGDEF domain-containing protein -
N5914_RS16045 (3333476) 3333476..3335824 - 2349 WP_022645413.1 EAL domain-containing protein -
N5914_RS16050 (3336001) 3336001..3336912 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14293.45 Da        Isoelectric Point: 9.9296

>T258923 WP_000539521.1 NZ_CP104665:3331365-3331751 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT258923 WP_001280945.1 NZ_CP104665:3331741-3332070 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GCT6

Antitoxin

Source ID Structure

References