Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3029949..3030733 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag N5914_RS14530 Protein ID WP_000613626.1
Coordinates 3030239..3030733 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag N5914_RS14525 Protein ID WP_024946541.1
Coordinates 3029949..3030242 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS14515 (3025110) 3025110..3026069 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
N5914_RS14520 (3026642) 3026642..3029815 + 3174 WP_022645526.1 ribonuclease E -
N5914_RS14525 (3029949) 3029949..3030242 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
N5914_RS14530 (3030239) 3030239..3030733 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
N5914_RS14535 (3030828) 3030828..3031781 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
N5914_RS14540 (3031793) 3031793..3033436 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
N5914_RS14545 (3033502) 3033502..3034443 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
N5914_RS14550 (3034443) 3034443..3035540 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T258922 WP_000613626.1 NZ_CP104665:3030239-3030733 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT258922 WP_024946541.1 NZ_CP104665:3029949-3030242 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References