Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1142679..1143262 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag N5914_RS05460 Protein ID WP_000254750.1
Coordinates 1142927..1143262 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag N5914_RS05455 Protein ID WP_000581937.1
Coordinates 1142679..1142927 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS05445 (1139018) 1139018..1140319 + 1302 WP_000046818.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
N5914_RS05450 (1140367) 1140367..1142601 + 2235 WP_000226815.1 GTP pyrophosphokinase -
N5914_RS05455 (1142679) 1142679..1142927 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N5914_RS05460 (1142927) 1142927..1143262 + 336 WP_000254750.1 endoribonuclease MazF Toxin
N5914_RS05465 (1143334) 1143334..1144125 + 792 WP_001071641.1 nucleoside triphosphate pyrophosphohydrolase -
N5914_RS05470 (1144353) 1144353..1145990 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N5914_RS05475 (1146077) 1146077..1147375 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T258916 WP_000254750.1 NZ_CP104665:1142927-1143262 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT258916 WP_000581937.1 NZ_CP104665:1142679-1142927 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References