Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 39737..40473 Replicon plasmid unnamed1
Accession NZ_CP104661
Organism Klebsiella pneumoniae strain 2021CK-01729

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag N4T33_RS26020 Protein ID WP_003026803.1
Coordinates 39991..40473 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag N4T33_RS26015 Protein ID WP_003026799.1
Coordinates 39737..40003 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T33_RS26005 (N4T33_26010) 35719..36297 - 579 WP_004206571.1 recombinase family protein -
N4T33_RS26010 (N4T33_26015) 36456..39440 + 2985 Protein_40 Tn3 family transposase -
N4T33_RS26015 (N4T33_26020) 39737..40003 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
N4T33_RS26020 (N4T33_26025) 39991..40473 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
N4T33_RS26025 (N4T33_26030) 40674..42077 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
N4T33_RS26030 (N4T33_26035) 42106..42738 - 633 WP_001567369.1 hypothetical protein -
N4T33_RS26035 (N4T33_26040) 43020..43277 - 258 WP_023292103.1 hypothetical protein -
N4T33_RS26040 (N4T33_26045) 43755..44483 + 729 Protein_46 CSS-motif domain-containing protein -
N4T33_RS26045 (N4T33_26050) 44499..45335 + 837 WP_032409249.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186135 186135
- inside IScluster/Tn - - 35719..42077 6358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T258910 WP_003026803.1 NZ_CP104661:39991-40473 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT258910 WP_003026799.1 NZ_CP104661:39737-40003 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References