Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4643924..4644734 Replicon chromosome
Accession NZ_CP104659
Organism Klebsiella pneumoniae strain 2020CK-00096

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag N5936_RS22555 Protein ID WP_004178461.1
Coordinates 4644201..4644734 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N5936_RS22550 Protein ID WP_002887278.1
Coordinates 4643924..4644190 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5936_RS22530 (4639795) 4639795..4640139 - 345 WP_004894569.1 cation efflux system protein CusF -
N5936_RS22535 (4640157) 4640157..4641542 - 1386 WP_026005908.1 efflux transporter outer membrane subunit -
N5936_RS22540 (4641715) 4641715..4642398 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
N5936_RS22545 (4642388) 4642388..4643821 + 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
N5936_RS22550 (4643924) 4643924..4644190 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N5936_RS22555 (4644201) 4644201..4644734 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
N5936_RS22560 (4644782) 4644782..4645903 - 1122 WP_004186850.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T258894 WP_004178461.1 NZ_CP104659:4644201-4644734 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT258894 WP_002887278.1 NZ_CP104659:4643924-4644190 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References