Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4078609..4079234 Replicon chromosome
Accession NZ_CP104659
Organism Klebsiella pneumoniae strain 2020CK-00096

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N5936_RS19915 Protein ID WP_002882817.1
Coordinates 4078851..4079234 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N5936_RS19910 Protein ID WP_004150355.1
Coordinates 4078609..4078851 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5936_RS19885 (4074600) 4074600..4075199 + 600 WP_004151865.1 glucose-1-phosphatase -
N5936_RS19890 (4075193) 4075193..4076053 + 861 WP_004146232.1 virulence factor BrkB family protein -
N5936_RS19895 (4076050) 4076050..4076487 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N5936_RS19900 (4076532) 4076532..4077473 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N5936_RS19905 (4077487) 4077487..4078404 - 918 WP_009484979.1 alpha/beta hydrolase -
N5936_RS19910 (4078609) 4078609..4078851 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N5936_RS19915 (4078851) 4078851..4079234 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N5936_RS19920 (4079408) 4079408..4080337 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N5936_RS19925 (4080334) 4080334..4080969 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N5936_RS19930 (4080966) 4080966..4081868 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T258890 WP_002882817.1 NZ_CP104659:4078851-4079234 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT258890 WP_004150355.1 NZ_CP104659:4078609-4078851 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References