258878

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 91531..92119 Replicon plasmid unnamed2
Accession NZ_CP104653
Organism Acinetobacter variabilis strain 2021CK-01005

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag N5980_RS16830 Protein ID WP_000438825.1
Coordinates 91531..91818 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag N5980_RS16835 Protein ID WP_004728120.1
Coordinates 91805..92119 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5980_RS16810 (N5980_16795) 87535..88314 + 780 WP_000422636.1 aminoglycoside O-phosphotransferase APH(3')-VIa -
N5980_RS16815 (N5980_16800) 88451..89419 + 969 WP_012308653.1 IS30 family transposase -
N5980_RS16820 (N5980_16805) 89416..89814 + 399 WP_080768162.1 LysE family transporter -
N5980_RS16825 (N5980_16810) 89949..91274 + 1326 WP_234622163.1 ISNCY family transposase -
N5980_RS16830 (N5980_16815) 91531..91818 + 288 WP_000438825.1 BrnT family toxin Toxin
N5980_RS16835 (N5980_16820) 91805..92119 + 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
N5980_RS16840 (N5980_16825) 92208..92738 - 531 WP_000172759.1 hypothetical protein -
N5980_RS16845 (N5980_16830) 92887..93189 + 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
N5980_RS16850 (N5980_16835) 93190..93471 + 282 WP_000985609.1 putative addiction module antidote protein -
N5980_RS16855 (N5980_16840) 93571..94779 + 1209 WP_042090285.1 IS256 family transposase -
N5980_RS16860 (N5980_16845) 94890..96215 - 1326 WP_234622163.1 ISNCY family transposase -
N5980_RS16865 (N5980_16850) 96376..96795 - 420 WP_001180106.1 SMI1/KNR4 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-58 / blaNDM-1 / aph(3')-VIa / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / aac(3)-IId - 1..123124 123124
- inside IScluster/Tn aph(3')-VIa / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / aac(3)-IId - 86409..116064 29655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T258878 WP_000438825.1 NZ_CP104653:91531-91818 [Acinetobacter variabilis]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT258878 WP_004728120.1 NZ_CP104653:91805-92119 [Acinetobacter variabilis]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References