Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4332687..4333237 Replicon chromosome
Accession NZ_CP104649
Organism Escherichia coli strain PNUSAE008512

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag N5913_RS22040 Protein ID WP_001160975.1
Coordinates 4332923..4333237 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag N5913_RS22035 Protein ID WP_000125566.1
Coordinates 4332687..4332920 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5913_RS22010 (4327761) 4327761..4328048 + 288 WP_000203741.1 ferredoxin-like protein FixX -
N5913_RS22015 (4328106) 4328106..4329437 + 1332 WP_001183177.1 MFS transporter -
N5913_RS22020 (4329545) 4329545..4330075 + 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
N5913_RS22025 (4330068) 4330068..4331930 + 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
N5913_RS22030 (4332122) 4332122..4332601 + 480 WP_000624372.1 type 3 dihydrofolate reductase -
N5913_RS22035 (4332687) 4332687..4332920 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
N5913_RS22040 (4332923) 4332923..4333237 + 315 WP_001160975.1 CcdB family protein Toxin
N5913_RS22045 (4333234) 4333234..4334082 - 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
N5913_RS22050 (4334089) 4334089..4334466 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
N5913_RS22055 (4334469) 4334469..4335290 - 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
N5913_RS22060 (4335287) 4335287..4336276 - 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
N5913_RS22065 (4336276) 4336276..4337562 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T258868 WP_001160975.1 NZ_CP104649:4332923-4333237 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT258868 WP_000125566.1 NZ_CP104649:4332687-4332920 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References